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分离绿色荧光蛋白系统可视化细菌在感染过程中传递的效应
JoVE Journal
Biology
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JoVE Journal Biology
Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection
DOI:

07:25 min

May 24, 2018

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Chapters

  • 00:04Title
  • 00:49Preparation of Plant Materials and Pseudomonas Culture
  • 02:09Transient Expression of Organelle-targeted sfGFP1-10OPT in N. benthamiana
  • 03:58Inoculation of Pseudomonas and Observation of sfGFP Signal via Confocal Microscopy
  • 05:46Results: Split Superfolder Fluorescent Protein System: A Useful Tool to Examine the Dynamics of Effectors in Host Plants
  • 06:32Conclusion

Summary

Automatic Translation

以荧光蛋白为基础的方法来监测细菌分泌到宿主细胞中的效应, 这是很有挑战性的。这是由于荧光蛋白与 III. 型分泌系统之间的不相容。在这里, 一个优化的分裂 superfolder GFP 系统是用来可视化的效应, 细菌分泌的宿主植物细胞。

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