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JoVE Journal
Biology

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分离绿色荧光蛋白系统可视化细菌在感染过程中传递的效应
 
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分离绿色荧光蛋白系统可视化细菌在感染过程中传递的效应

Article DOI: 10.3791/57719
May 24th, 2018

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Summary May 24th, 2018

Please note that all translations are automatically generated.

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以荧光蛋白为基础的方法来监测细菌分泌到宿主细胞中的效应, 这是很有挑战性的。这是由于荧光蛋白与 III. 型分泌系统之间的不相容。在这里, 一个优化的分裂 superfolder GFP 系统是用来可视化的效应, 细菌分泌的宿主植物细胞。

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