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Journal / Biochemistry / その場で近接結紮アッセイを用いたタンパク質アイソ フォームの Heterodimerization …
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Biochemistry
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JoVE Journal Biochemistry
その場で近接結紮アッセイを用いたタンパク質アイソ フォームの Heterodimerization の検出
Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
Journal (Biochemistry)
Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
Journal (Biochemistry)
Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
 
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その場で近接結紮アッセイを用いたタンパク質アイソ フォームの Heterodimerization の検出

Article DOI: 10.3791/57755-v • 09:18 min • October 20th, 2018
October 20th, 2018 •
1, 1
1Fox Chase Cancer Center

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ここでは、近接結紮の試金 (PLA) を使用して感度が高い固定細胞における MST1/MST2 heterodimerization を視覚化する方法を示します。

Tags

生化学、問題 140、PLA、MST、シグナル伝達、二量体化、タンパク質間相互作用、カバ経路
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