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吞噬细胞内复制脓肿分枝杆菌毒力标志物的鉴定
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Immunology and Infection
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JoVE Journal Immunology and Infection
Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes
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吞噬细胞内复制脓肿分枝杆菌毒力标志物的鉴定

DOI:
10.3791/57766-v

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08:34 min

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September 27, 2018

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, , , , , ,
12I, UVSQ, INSERM UMR1173,Université Paris-Saclay, 2Institut Pasteur,Unité de Pathogénomique Mycobactérienne

Chapters

  • 00:04Title
  • 00:46Amoebae: M. Abscessus Tn Mutant Co-culture
  • 03:32Intracellular Mycobacteria RNA Extraction for RNAseq Analysis
  • 07:18Results: Mycobacterial mRNA Extraction Analysis
  • 07:47Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出两个协议来研究吞噬-分枝杆菌脓肿的相互作用: 座子突变文库的筛选和 RNA 中细菌细胞内转录的测定测 序。这两种方法都提供了对基因组优势和 transcriptomic 适应增强细胞内细菌适应性的洞察力。

Tags

Mycobacterium AbscessusVirulence MarkersIntracellular ReplicationPhagocytesMutant Library ScreeningTranscriptomic StudiesAcanthamoeba CastellaniAmoebaCo-cultureAmikacinEscherichia ColiCell LysisColumbia Agar Plates

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