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Identifikation af virulens markører af Mycobacterium abscessus intracellulære replikering i fagocytter
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes
DOI:

08:34 min

September 27, 2018

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Chapters

  • 00:04Title
  • 00:46Amoebae: M. Abscessus Tn Mutant Co-culture
  • 03:32Intracellular Mycobacteria RNA Extraction for RNAseq Analysis
  • 07:18Results: Mycobacterial mRNA Extraction Analysis
  • 07:47Conclusion

Summary

Automatic Translation

Her præsenterer vi to protokoller for at studere phagocyt -Mycobacterium abscessus interaktioner: screening af en transposon mutant bibliotek for bakteriel intracellulære mangel og bestemmelse af bakteriel intracellulære transkriptom fra RNA sekventering. Begge tilgange giver indsigt i genomisk fordele og transkriptom tilpasninger forbedre intracellulære bakterier fitness.

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