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Cancer Research
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腺病毒报告系统 TGF β/Smad3 信号通路的活体
细胞成像
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Cancer Research
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JoVE Journal
Cancer Research
Live Cell Imaging of the TGF- β/Smad3 Signaling Pathway
In Vitro
and
In Vivo
Using an Adenovirus Reporter System
Please note that all translations are automatically generated.
Click here for the English version.
腺病毒报告系统 TGF β/Smad3 信号通路的活体
细胞成像
DOI:
10.3791/57926-v
•
11:06 min
•
July 30, 2018
•
Hao Chen*
1
,
Thomas M.B. Ware*
1
,
Josephine Iaria
,
Hong-Jian Zhu
1
Department of Surgery (RMH)
,
University of Melbourne
Chapters
00:04
Title
00:53
Dual-adenovirus Transduction in Live Single Cells
02:32
Live Single Cell Signaling in Wound Healing Assay
04:16
Cell Preparation for Live Signaling Imaging
In Vivo
06:49
Orthotopic Implantation
08:28
Results: Live Single Cell Imaging
In Vitro
and Live TGF-β Signaling Imaging
In Vivo
10:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了一个使用腺病毒记者系统的 TGF-β/Smad3 信号活动的活细胞成像协议。该系统实时跟踪转录活性, 可用于
体外
和活体动物模型中的单细胞
.
Tags
Live Cell Imaging
TGF-β/Smad3 Signaling Pathway
Adenovirus Reporter System
Cancer Cell Lines
Breast Cancer
Wound Healing Assay
MDA-MB-231 Cells
Adenovirus Infection
Fluorescence Microscopy
GFP
Td-Tomato
TGF-beta
ImageJ
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