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成像 FITC-葡聚糖作为胞吐作用调节的记者
JoVE Journal
Biology
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JoVE Journal
Biology
Imaging FITC-dextran as a Reporter for Regulated Exocytosis
Please note that all translations are automatically generated.
Click here for the English version.
成像 FITC-葡聚糖作为胞吐作用调节的记者
DOI:
10.3791/57936-v
•
04:50 min
•
June 20, 2018
•
Ofir Klein
,
Amit Roded
,
Koret Hirschberg
,
Mitsunori Fukuda
,
Stephen J. Galli
,
Ronit Sagi-Eisenberg
1
Department of Cell and Developmental Biology, Sackler Faculty of Medicine
,
Tel Aviv University
,
2
Department of Pathology, Sackler Faculty of Medicine
,
Tel Aviv University
,
3
Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences
,
Tohoku University
,
4
Departments of Pathology and of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, School of Medicine
,
Stanford University
Chapters
00:04
Title
00:34
Preparations and Culture of RBL Cells for Live Cell Microscopy
01:52
Live Cell Microscopy of Exocytosis
03:43
Results: FITC-dextran May Act as a Reporter for Regulated Exocytosis
04:17
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们详细介绍了一种带电细胞成像的调节胞吐作用方法。该方法利用 FITC-葡聚糖, 在溶酶体相关细胞器中积累, 作为记者。这一简单的方法也允许区分不同模式的调节胞吐作用在细胞难以操纵基因。
Tags
FITC-dextran
Regulated Exocytosis
Mast Cell Exocytosis
Neutrophils
Eosinophils
Tyrode’s Buffer
RBL Cells
Ammonium Chloride
Secretagogue
Fluorescence Microscopy
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