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Imagem latente FITC-dextrano como repórter por exocitose regulada

Article DOI: 10.3791/57936 • 04:50 min • June 20th, 2018
June 20th, 2018 •
1, 1, 2, 3, 4, 1
1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, 2Department of Pathology, Sackler Faculty of Medicine, Tel Aviv University, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, 4Departments of Pathology and of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, School of Medicine, Stanford University

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Aqui detalhamos um método para a imagem latente de célula viva de exocitose regulada. Este método utiliza FITC-dextrano, que acumula em organelas lisossoma-relacionados, como repórter. Este método simples também permite distinguir entre diferentes modos de exocitose regulada em células que são difíceis de manipular geneticamente.

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Biologia questão 136 FITC-dextrano exocitose regulamentado composto de exocitose imagem de célula viva organelas relacionadas com lisossomo pinocitose
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