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1Department of Cell and Developmental Biology, Sackler Faculty of Medicine,Tel Aviv University, 2Department of Pathology, Sackler Faculty of Medicine,Tel Aviv University, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences,Tohoku University, 4Departments of Pathology and of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, School of Medicine,Stanford University
Chapters
- 00:04Title
- 00:34Preparations and Culture of RBL Cells for Live Cell Microscopy
- 01:52Live Cell Microscopy of Exocytosis
- 03:43Results: FITC-dextran May Act as a Reporter for Regulated Exocytosis
- 04:17Conclusion
Här detalj vi en metod för levande cell avbildning av reglerade exocytos. Denna metod använder FITC-dextran, som ackumuleras i lysosomen-relaterade organeller, som reporter. Den här enkla metoden kan också skilja mellan olika lägen av reglerade exocytos i celler som är svåra att manipulera genetiskt.
