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1Department of Cell and Developmental Biology, Sackler Faculty of Medicine,Tel Aviv University, 2Department of Pathology, Sackler Faculty of Medicine,Tel Aviv University, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences,Tohoku University, 4Departments of Pathology and of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, School of Medicine,Stanford University
Chapters
- 00:04Title
- 00:34Preparations and Culture of RBL Cells for Live Cell Microscopy
- 01:52Live Cell Microscopy of Exocytosis
- 03:43Results: FITC-dextran May Act as a Reporter for Regulated Exocytosis
- 04:17Conclusion
Burada biz ayrıntılı olarak düzenlenmiş ekzositozu canlı hücre görüntüleme için bir yöntem. Bu yöntem lysosome ilgili organelleri içinde birikir, FITC-dextran muhabir olarak kullanır. Bu basit yöntem aynı zamanda genetik olarak işlemek zor olan hücrelerdeki düzenlenmiş ekzositozu farklı modları arasında ayrım sağlar.
