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利用下一代序列染色体构象捕获 (4 c 序列) 的高通量识别基因调控数列
JoVE Journal
Genetics
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JoVE Journal Genetics
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
DOI:

09:06 min

October 05, 2018

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Chapters

  • 00:04Title
  • 00:45Formaldehyde Cross-linking and Cell Lysis
  • 01:59First Restriction Digestion and First Ligation
  • 05:07Cross-linking Reversion, Chromatin Isolation, and Further Analyses
  • 07:42Results: Representative Genomic Track of 4C-seq Read Coverage
  • 08:29Conclusion

Summary

Automatic Translation

基因和调控元素之间的物理相互作用的识别是具有挑战性的, 但已被染色体构象捕获方法所促进。这种对 4 c 序列协议的修改可以减少 pcr 模板的过度放大, 并通过加入限制酶消化步骤来最大限度地提高 mappability 的读数。

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