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JoVE Journal
Neuroscience

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Overexpressing 突触前蛋白培养神经元突触囊泡再生的光学检测
 
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Overexpressing 突触前蛋白培养神经元突触囊泡再生的光学检测

Article DOI: 10.3791/58043
June 26th, 2018

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Summary June 26th, 2018

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我们描述了一种用于培养神经元的突触囊泡 (SV) 循环的光学检测方法。将该协议与双转染相结合, 以表达突触前标记和感兴趣的蛋白质, 使我们能够定位突触前的部位, 它们的突触囊泡再生能力, 并确定感兴趣的蛋白质的作用。

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