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Chapters
- 00:04Title
- 00:37Adult Zebrafish Ventricular Resection
- 02:42Preparation of siRNA-encapsulated Nanoparticles and Injection of siRNA-encapsulated Nanoparticles into Adult Zebrafish Heart
- 04:29Heart Collection, Fixation, and Evaluation of the Efficiency of siRNA Delivery
- 05:34Evaluation of Nanoparticle-mediated siRNA Gene-silencing
- 06:38Results: Nanoparticle-mediated siRNA Gene-silencing
- 08:01Conclusion
Det er fortsatt en stor utfordring å utvikle betinget gen-knockout eller effektiv gen-knockdown i voksen sebrafisk organer. Her kan vi rapportere en protokoll for utøvende hydrogenion-mediert siRNA gen-stanse i voksen sebrafisk hjerte, noe som gir en ny tap-av-funksjonen metoden for å studere voksen organer i sebrafisk og andre modellen organismer.
