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纳米粒子介导的 siRNA 基因沉默在斑马鱼心脏中的表达
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Developmental Biology
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JoVE Journal Developmental Biology
Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart
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纳米粒子介导的 siRNA 基因沉默在斑马鱼心脏中的表达

DOI:
10.3791/58054-v

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09:12 min

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July 29, 2018

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, , , , ,
1Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine,Peking University, 2Department of Biomedical Engineering, College of Engineering,Peking University

Chapters

  • 00:04Title
  • 00:37Adult Zebrafish Ventricular Resection
  • 02:42Preparation of siRNA-encapsulated Nanoparticles and Injection of siRNA-encapsulated Nanoparticles into Adult Zebrafish Heart
  • 04:29Heart Collection, Fixation, and Evaluation of the Efficiency of siRNA Delivery
  • 05:34Evaluation of Nanoparticle-mediated siRNA Gene-silencing
  • 06:38Results: Nanoparticle-mediated siRNA Gene-silencing
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

在成年斑马鱼器官中发展有条件基因剔除或有效基因击倒仍是一项重大挑战。在这里, 我们报告了在成年斑马鱼心脏执行纳米粒子介导的 siRNA 基因沉默的协议, 从而为研究斑马鱼和其他模型生物体中的成年器官提供了一种新的功能损失方法。

Tags

NanoparticleSiRNAGene-silencingZebrafishHeart RegenerationVentricular ResectionPAM Nanoparticle DendrimerALDH1A2 SiRNAScrambled Negative Control SiRNACyanine DyesAnesthesiaSurgeryRecovery

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