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Journal / Developmental Biology / Nanopartikler-medieret siRNA genhæmning i voksen…
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Developmental Biology

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JoVE Journal Developmental Biology
Nanopartikler-medieret siRNA genhæmning i voksen zebrafisk hjerte
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Nanopartikler-medieret siRNA genhæmning i voksen zebrafisk hjerte

Article DOI: 10.3791/58054-v • 09:12 min • July 29th, 2018
July 29th, 2018 •
1, 2, 1, 1, 2, 1
1Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine, Peking University, 2Department of Biomedical Engineering, College of Engineering, Peking University

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Det er fortsat en stor udfordring at udvikle betinget af gen-knockout eller effektiv af gen-knockdown i voksen zebrafisk organer. Her rapporterer vi en protokol for udfører nanopartikel-medieret siRNA genhæmning i voksen zebrafisk hjertet, hvilket giver en ny tab af funktion metode til at studere voksen organer i zebrafisk og andre modelorganismer.

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Udviklingsmæssige biologi sag 137 zebrafisk voksen hjerte regenerering nanopartikler siRNA gene silencing udviklingsmæssige biologi genetik
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