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Biochemistry
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バイオアッセイ誘導分別を使用して海ヤツメウナギ フェロモンの同定
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation
Please note that all translations are automatically generated.
Click here for the English version.
バイオアッセイ誘導分別を使用して海ヤツメウナギ フェロモンの同定
DOI:
10.3791/58059-v
•
09:35 min
•
July 17, 2018
•
Anne M. Scott
,
Ke Li
,
Weiming Li
1
Department of Fisheries and Wildlife
,
Michigan State University
Chapters
00:04
Title
00:27
Collection and Extraction of Sea Lamprey Conditioned Water
01:14
Isolation of Fraction Pools with Chromatography
02:51
Electro-olfactogram Recordings to Identify Odorous Fractions/Compounds
05:43
Two-choice Maze Behavioral Bioassay to Identify Behaviorally Active Fractions/Compounds
06:31
Chromatographic Isolation of Pure Compounds from Active Fractions
06:58
Structure Elucidation of a Pure Compound with Mass Spectrometry and Nuclear Magnetic Resonance
07:58
Results: Behavioral Responses of Ovulated Female Sea Lampreys to Odorants
08:36
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、分離し、構造、嗅力海ヤツメウナギの推定フェロモン化合物の行動応答を評価するためのプロトコルを提案する.
Tags
Sea Lamprey
Pheromone Identification
Bioassay-guided Fractionation
Chemical Ecology
Solid-phase Extraction
Silica Gel Chromatography
Thin-layer Chromatography
Electro-olfactogram Recording
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