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体外犬中性粒细胞胞外陷阱的形成: 荧光显微镜动态定量分析
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy
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体外犬中性粒细胞胞外陷阱的形成: 荧光显微镜动态定量分析

DOI:
10.3791/58083-v

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09:45 min

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August 24, 2018

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1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine,University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine,University of California, Davis

Chapters

  • 00:04Title
  • 00:34Neutrophil Isolation
  • 03:03Live Neutrophil Imaging and Neutrophil Extracellular Trap (NET) Detection and Quantification
  • 07:18Results: Representative Canine Neutrophil Imaging and NET Detection
  • 08:55Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了从全血中分离犬中性粒细胞的方法, 用荧光显微镜观察活中性粒细胞的网状形成。还描述了使用免疫荧光显微镜定量分析网络形成和瓜组蛋白 H3 (citH3) 表达的协议。

Tags

In VitroCanineNeutrophilExtracellular TrapFormationFluorescence MicroscopyNETPeroxidaseCitrullinated Histone H3BloodJugular VeinAnticoagulantDextranDensity Gradient CentrifugationErythrocytesPolymorphonuclear CellsCell Culture BufferTrypan BluePoly-L-lysineFluorescence Microscopy

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