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JoVE Journal
Genetics
Author Produced

This content is Open Access.

Endogene Protein Tagging i menneskelige induceret pluripotente stamceller ved hjælp af CRISPR/Cas9
 
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Endogene Protein Tagging i menneskelige induceret pluripotente stamceller ved hjælp af CRISPR/Cas9

Article DOI: 10.3791/58130
August 25th, 2018

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Summary August 25th, 2018

Please note that all translations are automatically generated.

Click here for the English version.

Beskrevet her er en protokol til tagging endogent udtrykte proteiner med fluorescerende tags i menneskelige inducerede pluripotente stamceller ved hjælp af CRISPR/Cas9. Derfor redigerede celler er beriget af fluorescens aktiveret celle sortering og klonede cellelinjer genereres.

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