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JoVE Journal
Genetics
Author Produced

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Endogen Protein taggning i mänskliga inducerade pluripotenta stamceller med CRISPR/Cas9
 
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Endogen Protein taggning i mänskliga inducerade pluripotenta stamceller med CRISPR/Cas9

Article DOI: 10.3791/58130
August 25th, 2018

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Summary August 25th, 2018

Please note that all translations are automatically generated.

Click here for the English version.

Beskrivs här är ett protokoll för taggning endogenously uttryckte proteiner med fluorescerande Taggar i mänskliga inducerade pluripotenta stamceller med CRISPR/Cas9. Förment redigerade celler berikas av fluorescens aktiverad cell sortering och klonal cellinjer genereras.

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