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蜜蜂匀浆中磷脂酶 C 活性的检测

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee

Please note that all translations are automatically generated. Click here for the English version.

蜜蜂匀浆中磷脂酶 C 活性的检测

DOI:

10.3791/58173-v

•

08:30 min

•

September 14, 2018

•

Shota Suenami², Ryo Miyazaki, Takeo Kubo

¹Bioproduction Research Institute,National Institute of Advanced Industrial Science and Technology, ²Department of Biological Sciences, Graduate School of Science,The University of Tokyo

Chapters

00:04Title
00:39Dissection of the Honeybee Brain
02:59Preparation of Brain Homogenates
04:21PLC Reaction in Brain Homogenates
05:28Detection of Fluorescence Resulting from PLC Activity
05:56Results: PLC Activities With and Without PLC Inhibitors in the Mushroom Bodies and Other Brain Regions
07:25Conclusion

Summary

Automatic Translation

为了检验药理学药物对蜜蜂脑不同区域磷脂酶 C (plc) 的抑制作用, 我们提出了一种生化检测方法来测量这些地区的 plc 活性。该方法可用于比较组织中的 PLC 活动, 以及不同行为的蜜蜂之间的对比。

Tags

Phospholipase C Activity Brain Homogenate Honeybee Neuroscience Dissection Homogenization Enzyme Assay

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