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Journal
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Bioengineering
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基于网模的无支架三维心脏组织创建方法
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation
Please note that all translations are automatically generated.
Click here for the English version.
基于网模的无支架三维心脏组织创建方法
DOI:
10.3791/58252-v
•
06:57 min
•
August 05, 2018
•
Yang Bai
2
,
Enoch Yeung
,
Cecillia Lui
,
Chin Siang Ong
,
Isaree Pitaktong
,
Chenyu Huang
,
Takahiro Inoue
,
Hiroshi Matsushita
,
Chunye Ma
,
Narutoshi Hibino
1
Department of Cardiac Surgery
,
The First Hospital of Jilin University
,
2
Division of Cardiac Surgery
,
Johns Hopkins Hospital
Chapters
00:00
Title
00:14
Process Flow of Cardiac Tissue Creation by Novel Mold
01:21
Spheroids Creation by Hanging Drop Method
03:26
Seeding Spheroids into the Mold Cavity
05:16
Result: The 3D Cardiac Patch Contains of Stacked Spheroids with Functional Beating after Removing from Novel Mold
05:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了一种基于净模的方法来创建三维无支架的心脏组织, 结构完整性和同步跳动行为令人满意。
Tags
Scaffold-free
Spheroids
Hanging Drop System
Mold-based Method
Cardiomyocytes
Fibroblasts
Endothelial Cells
Reusable Device
Spontaneous Formation
Mechanical Integrity
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