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切片中石蜡嵌入组织的切割和漂浮方法

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning

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切片中石蜡嵌入组织的切割和漂浮方法

DOI:

10.3791/58288-v

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05:00 min

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September 05, 2018

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Cheng Qin*^1,2, Yijiang Bai*^1,2, Zhen Zeng², Liao Wang², Zhiwen Luo², Shunqi Wang³, Suqi Zou³

¹Institute of Life Science,Nanchang University, ²Queen Mary School, Medical Department,Nanchang University, ³School of Life Science,Nanchang University

Chapters

00:04Title
00:27Assemble the Equipment and Connect the Microtome
01:51Paraffin Sectioning
03:24Results: Analysis of the Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning
04:32Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个改进石蜡切片的协议。这种方法结合切割和浮动使用一个简单的恒温室, 以避免转让过程中所需的传统方法。结果表明, 石蜡切片的效率和数量均有显著提高。

Tags

Sectioning Cutting And Floating Method Fume Hood Acrylic Boards Chloroform Section Channel Water Bath Heating Element Digital Thermostat Microtome Neutral Silicone Sealant Paraffin Sectioning Temperature Detection Switch Paraffin Block Cassette Clamp Section Thickness Floating Sections Microscope Slide

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