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Journal
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Immunology and Infection
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利用超滤离心技术分离小鼠支气管肺泡灌洗液中的细胞外囊
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique
Please note that all translations are automatically generated.
Click here for the English version.
利用超滤离心技术分离小鼠支气管肺泡灌洗液中的细胞外囊
DOI:
10.3791/58310-v
•
09:18 min
•
November 09, 2018
•
Tanyalak Parimon
,
Norman E. Garrett III
,
Peter Chen
2
,
Travis J. Antes
1
Department of Medicine, Division of Pulmonary and Critical Care, Women’s Guild Lung Institute
,
Cedars-Sinai Medical Center
,
2
Department of Biomedical Sciences
,
Cedars-Sinai Medical Center
,
3
Department of Medicine, Smidt Heart Institute
,
Cedars-Sinai Medical Center
Chapters
00:04
Title
01:15
Murine Bronchoalveolar Lavage Fluid (BALF) Collection and Preparation
02:21
Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid
04:56
Extracellular Vesicle Quantification
05:35
Detection of BALF-derived Extracellular Vesicles
07:16
Results: UFC Method Demonstrates Higher Total Particle Counts and Protein Recover
08:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们描述了两个细胞外囊泡分离方案, 超滤离心和超离心与密度梯度离心, 从小鼠支气管肺泡灌洗液样品中分离细胞外囊泡。用两种方法对小鼠支气管肺泡灌洗液中的细胞外囊泡进行了定量和表征。
Tags
Extracellular Vesicles
Bronchoalveolar Lavage Fluid
Ultrafiltration Centrifugation
Ultracentrifugation
Density Gradient Purification
Murine
Trachea
DPBS
Supernatant
Syringe Filter
Centrifugal Filter Unit
Retentate
Aliquot
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