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激光辅助慢病毒基因传递到小鼠受精卵
JoVE Journal
Biology
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JoVE Journal
Biology
Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs
Please note that all translations are automatically generated.
Click here for the English version.
激光辅助慢病毒基因传递到小鼠受精卵
DOI:
10.3791/58327-v
•
06:03 min
•
November 01, 2018
•
Negin P. Martin*
1
,
Page Myers*
2
,
Eugenia Goulding
,
Shih-Heng Chen
,
Mitzie Walker
,
Thomas M. Porter
,
Lucas Van Gorder
,
Amanda Mathew
,
Artiom Gruzdev
,
Erica Scappini
,
Charles Romeo
1
Neurobiology Laboratory
,
National Institute of Environmental Health Sciences
,
2
Comparative Medicine Branch
,
National Institute of Environmental Health Sciences
,
3
Reproductive and Developmental Biology Laboratory
,
National Institute of Environmental Health Sciences
,
4
Signal Transduction Laboratory
,
National Institute of Environmental Health Sciences
Chapters
00:04
Title
00:55
Mouse Fertilized Egg Isolation
02:12
Mouse Fertilized Eggs XYClone Laser Perforation
03:13
Mouse Fertilized Egg Transduction and Non-surgical Transfer
04:00
Results: Representative Lentivirus Transduction Analyses
04:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
小鼠受精卵和早期胚胎受透明带保护, 透明带是一种糖蛋白基质, 形成了对抗基因传递的屏障。本文介绍了一种用激光射孔带带的协议, 用慢病毒载体传递胚胎细胞, 并创造转基因小鼠。
Tags
Laser-assisted
Lentiviral
Gene Delivery
Fertilized Eggs
Mouse
Zona Pellucida
Perforation
Micromanipulation
Microinjection
Ovaries
Ovaducts
Cumulus Cells
KSOM
XYClone Laser
LED
Zona Pellucida Perforation
Article
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