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Laser-assisteret Lentiviral gen levering til musen befrugtede æg
 
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Laser-assisteret Lentiviral gen levering til musen befrugtede æg

Article DOI: 10.3791/58327
November 1st, 2018

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Summary November 1st, 2018

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Mus befrugtede æg og tidlige fase embryoner er beskyttet af zona pellucida, et glykoprotein matrix, der danner en barriere mod gen levering. I denne artikel beskrives en protokol for perforering af zona med en laser transduce embryonale celler med lentiviral vektorer og skabe Transgene mus.

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