A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Neurobiology Laboratory,National Institute of Environmental Health Sciences, 2Comparative Medicine Branch,National Institute of Environmental Health Sciences, 3Reproductive and Developmental Biology Laboratory,National Institute of Environmental Health Sciences, 4Signal Transduction Laboratory,National Institute of Environmental Health Sciences
Chapters
- 00:04Title
- 00:55Mouse Fertilized Egg Isolation
- 02:12Mouse Fertilized Eggs XYClone Laser Perforation
- 03:13Mouse Fertilized Egg Transduction and Non-surgical Transfer
- 04:00Results: Representative Lentivirus Transduction Analyses
- 04:55Conclusion
Mus befrugtede æg og tidlige fase embryoner er beskyttet af zona pellucida, et glykoprotein matrix, der danner en barriere mod gen levering. I denne artikel beskrives en protokol for perforering af zona med en laser transduce embryonale celler med lentiviral vektorer og skabe Transgene mus.
