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原发性鸡初囊-细胞培养模型研究传染性囊内病病毒发病机制
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
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原发性鸡初囊-细胞培养模型研究传染性囊内病病毒发病机制

DOI:
10.3791/58489-v

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07:26 min

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October 04, 2018

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, , , ,
1The Pirbright Institute

Chapters

  • 00:04Title
  • 00:45Isolation of Chicken Primary Bursal Cells
  • 02:59Culture of Chicken Primary Bursal Cells
  • 03:51Infection of Chicken Primary Bursal Cells with IBDV and Quantification of IBDV Replication
  • 05:02Results: Analysis of Chicken Primary Bursal Cell Culture and Infection with Infectious Bursal Disease Virus
  • 06:41Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们描述了鸡初囊细胞的分离, 从囊中, 感染的文化和感染的囊腔病病毒的细胞, 以及病毒复制的量化。

Tags

Keywords: Ex VivoChickenPrimary Bursal-cell CultureInfectious Bursal Disease VirusPathogenesisAvian VirologyB CellsImmortalized Cell LinesThree RsPBSCollagenase DHBBSRPMIFBSDensity Gradient MediaPolysucroseSodium Diatrizoate

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