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Immunology and Infection

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JoVE Journal Immunology and Infection
En rensning og In Vitro aktivitet Assay for en (p) ppGpp syntetase fra Clostridium difficile
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En rensning og In Vitro aktivitet Assay for en (p) ppGpp syntetase fra Clostridium difficile

Article DOI: 10.3791/58547-v • 09:53 min • November 3rd, 2018
November 3rd, 2018 •
1, 1, 1
1Department of Chemistry and Biochemistry, Old Dominion University

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Her, beskriver vi en metode til rensning af histidin-markeret pyrophosphokinase enzymer og anvende tyndtlagskromatografi radioaktive substrater og produkter til assay for enzymatisk aktivitet in vitro. Enzym aktivitet assay er stort set gælder for enhver kinase, nukleotid cyclase eller fosfor-overførsel reaktion hvis mekanisme omfatter nukleotid trifosfat hydrolyse.

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Immunologi og infektion udstede 141 Protein oprensning histidin tag (p) ppGpp tyndtlagskromatografi radionukleid mærkning pyrophosphokinase phosphotransfer
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