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Journal
/
Biochemistry
/
コナガ
からのリアノジン受容体の N 末端ドメインの結晶構造
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Crystal Structure of the N-terminal Domain of Ryanodine Receptor from
Plutella xylostella
Please note that all translations are automatically generated.
Click here for the English version.
コナガ
からのリアノジン受容体の N 末端ドメインの結晶構造
DOI:
10.3791/58568-v
•
11:31 min
•
November 30, 2018
•
Bidhan Chandra Nayak*
1
,
Jie Wang*
1
,
Lianyun Lin*
1,2,3,4
,
Weiyi He
3,4
,
Minsheng You
3,4
,
Zhiguang Yuchi
2
1
Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, Collaborative Innovation Center of Chemical Science and Engineering, School of Pharmaceutical Science and Technology
,
Tianjin University
,
2
State Key Laboratory of Ecological Pest Control for Fujian/Taiwan Crops and Institute of Applied Ecology
,
Fujian Agriculture and Forestry University
,
3
Joint International Research Laboratory of Ecological Pest Control
,
Ministry of Education, Fuzhou
,
4
Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests
,
Fujian Agriculture and Forestry University
Chapters
00:04
Title
01:01
Ligation-independent Cloning (LIC)
02:38
E. coli
Transformation and Protein Expression
04:58
Fusion Protein Purification, Tag-cleaving, and Target Protein Re-purification
06:12
Protein Crystallization, Crystal Mounting, and Structure Determination
09:56
Results: Representative N-terminal Domain (NTD) Crystal Structure Determination
10:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
この記事で私たちはコナガ (
コナガ
) からリアノジン受容体の N 末端ドメインの蛋白質の発現、精製、結晶化、構造決定のプロトコルを説明します。
Tags
Ryanodine Receptor
Crystal Structure
Insecticide
Insect
Protein Structure
X-ray Crystallography
Protein Crystallography
Polymerase Chain Reaction
DNA Amplification
LIC Vector
T4 DNA Polymerase
Ligation-independent Cloning
BL21(DE3) E. Coli
Protein Expression
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