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Journal / Neuroscience / Biocytin utvinning og 3D rekonstruksjoner av fylt …
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Neuroscience

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JoVE Journal Neuroscience
Biocytin utvinning og 3D rekonstruksjoner av fylt hippocampus CA2 Interneurons
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Biocytin utvinning og 3D rekonstruksjoner av fylt hippocampus CA2 Interneurons

Article DOI: 10.3791/58592-v • 11:21 min • November 20th, 2018
November 20th, 2018 •
*1, *1, 1
1Department of Pharmacology, University College London
* These authors contributed equally

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Click here for the English version.

Protokollen skissert her beskriver immunofluorescence analyse, biocytin utvinning og høy kvalitet rekonstruksjoner av hippocampus CA2 interneurons etter intracellulær elektrofysiologiske opptak i vitro, slik neuronal karakterisering og til slutt fine neuronal anatomi studier.

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Nevrovitenskap problemet 141 Immunohistochemistry immunofluorescence-protokollen HRP-protokollen neuronal rekonstruksjoner Neurolucida kamera Lucida neuronal anatomi dendrites axon
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