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Biocytin 回復と塗りつぶされた海馬 ca 2 介在ニューロンの 3 D 再構成
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons
DOI:

11:21 min

November 20, 2018

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Chapters

  • 00:04Title
  • 00:26Tissue Preparation and Staining for Fluorescence Microscopy and 3D Neuronal Reconstruction
  • 04:08Histological Detection of Biotin-filled Neurons
  • 08:033D Neuronal Reconstructions
  • 10:12Results: Reconstruction of a Basket Cell Recorded and Filled in the Hippocampal CA2 Region and Morphometry Analyses
  • 10:57Conclusion

Summary

Automatic Translation

ここで説明したプロトコルを記述する蛍光分析、biocytin 回復と海馬 ca 2 介在神経細胞内の電気生理学的記録in vitro での許可神経以下の質の高い復元特性と調査されるべき最終的に細かい神経解剖学。

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