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Biocytin inddrivelse og 3D rekonstruktioner af fyldt hippocampus CA2 Interneurons
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Chapters
- 00:04Title
- 00:26Tissue Preparation and Staining for Fluorescence Microscopy and 3D Neuronal Reconstruction
- 04:08Histological Detection of Biotin-filled Neurons
- 08:033D Neuronal Reconstructions
- 10:12Results: Reconstruction of a Basket Cell Recorded and Filled in the Hippocampal CA2 Region and Morphometry Analyses
- 10:57Conclusion
Den protokol, der er skitseret her beskriver immunofluorescens analyse, biocytin opsving og høj kvalitet rekonstruktioner af hippocampus CA2 interneurons efter den intracellulære elektrofysiologiske optagelser in vitro tillader neuronal karakterisering og i sidste ende fine neuronal anatomi undersøges.
