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Journal
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Biochemistry
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利用网格和收集荧光蛋白陶瓷的结构解
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
Please note that all translations are automatically generated.
Click here for the English version.
利用网格和收集荧光蛋白陶瓷的结构解
DOI:
10.3791/58594-v
•
06:42 min
•
March 19, 2019
•
Stephanie Hutin*
1
,
Gianluca Santoni*
1
,
Ulrich Zander
,
Nicolas Foos
,
Sylvain Aumonier
,
Guillaume Gotthard
,
Antoine Royant
3
,
Christoph Mueller-Dieckmann
,
Gordon Leonard
1
European Synchrotron Radiation Facility
,
Structural Biology Group
,
2
European Molecular Biology Laboratory
,
3
Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale)
Chapters
00:04
Title
00:51
Offline Preparation of the Synchrotron Experiment
01:31
Loading of the Sample onto a Beamline
03:49
Prepare and Execute the MeshAndCollect Workflow
05:02
Results: Structure Determination of the Fluorescent Protein Cerulean using MeshAndCollect
06:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们介绍了使用网格德德收集协议来获得一个完整的衍射数据集, 用于随后的结构测定, 由从荧光蛋白 Cerulean 的许多小晶体中收集的部分衍射数据集组成。
Tags
MeshAndCollect
Serial Crystallography
Rotation Data Collection
Small Crystals
Puck Samples
Structure Solution
Ligand Screening
Synchrotron Beamline
VMX
Protein Crystallography Beamline
Sample Changer
Data Collection
Visual Reorientation Workflow
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