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酸化浸渍法评价全肾肾素数
JoVE Journal
Medicine
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JoVE Journal
Medicine
Estimation of Nephron Number in Whole Kidney using the Acid Maceration Method
Please note that all translations are automatically generated.
Click here for the English version.
酸化浸渍法评价全肾肾素数
DOI:
10.3791/58599-v
•
08:15 min
•
May 22, 2019
•
Sarah M. Peterson
,
Xuexiang Wang
,
Ashley C. Johnson
,
Ian D. Coate
,
Michael R. Garrett
3
,
Sean P. Didion
4
1
Department of Pharmacology and Toxicology
,
The University of Mississippi Medical Center
,
2
Department of Medicine, Division of Nephrology
,
Rush University Medical Center
,
3
Department of Medicine, Division of Nephrology
,
The University of Mississippi Medical Center
,
4
Department of Neurology
,
The University of Mississippi Medical Center
Chapters
00:04
Title
01:34
Kidney Isolation
02:33
Kidney Homogenization, Incubation, and Straining
05:10
Glomeruli Enumeration and Nephron Number Extrapolation
06:20
Results: Representative Nephron Quantification
07:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
对整个肾肾的估计在临床和实验上都很重要, 因为肾周数与肾脏和心血管疾病的风险增加之间存在反比关系。在此, 说明了酸浸渍法的应用, 该方法为整个肾肾数目提供了快速可靠的估计。
Tags
Nephron Number
Acid Maceration Method
Kidney
Renal Function
Nephron Development
Gene Influence
Renal Pathology
Magnetic Resonance Imaging
Experimental Technique
Dissection
Kidney Weight
Hydrochloric Acid
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