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Microscopie 4D de levure

Article DOI: 10.3791/58618-v • 12:00 min • April 28th, 2019
April 28th, 2019 •
1, 1
1Department of Molecular Genetics and Cell Biology, University of Chicago

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Ce protocole décrit l'analyse des compartiments intracellulaires étiquetés fluorescents dans la levure en herbe utilisant la microscopie confocale 4D multicolore (time-lapse 3D). Les paramètres d'imagerie sont choisis pour capturer des signaux adéquats tout en limitant les dégâts photo. Les plugins Custom ImageJ permettent de suivre et d'analyser quantitativement les structures étiquetées.

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Biologie Numéro 146 Microscopie Confocal imagerie 4D fluorescence photoblanchiment déconvolution ImageJ levure
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