JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
酵母的4D显微镜
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
4D Microscopy of Yeast
Please note that all translations are automatically generated.
Click here for the English version.
酵母的4D显微镜
DOI:
10.3791/58618-v
•
12:00 min
•
April 28, 2019
•
Natalie Johnson
,
Benjamin S. Glick
1
Department of Molecular Genetics and Cell Biology
,
University of Chicago
Chapters
00:04
Title
00:35
Yeast Preparation
02:02
Imaging
04:54
Deconvolution and Bleach Correction
07:39
Movie Generation and Editing to Isolate and Analyze Individual Structures
10:09
Results: Representative 4-Dimensional (4D) Yeast Microscopy
11:24
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议描述了使用多色4D(延时3D)共聚焦显微镜对萌芽酵母中荧光标记细胞内腔的分析。选择成像参数是为了捕捉足够的信号,同时限制光损伤。自定义 ImageJ 插件允许跟踪和定量分析标记的结构。
Tags
AI
4D Microscopy
Yeast
Organelles
Intracellular Dynamics
Concanavalin A
Confocal Microscopy
Xyzt
Bidirectional X Scanning
Pixel Size
Airy Units
White Light Laser
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
从酵母细胞线粒体的纯化
酵母殖民地嵌入方法
在酵母用于监测Mitophagy荧光显微镜检测酿酒酵母
高通量酵母质粒过表达屏幕
高活性酵母核糖体的色谱分离纯化
裂殖酵母的活细胞定量荧光显微镜分析
在活细胞中蛋白质聚集的4D成像
使用4D共聚焦显微镜分析颅面形态的斑马鱼
表面扩散和酵母染色体免疫染色
裂殖酵母性生命周期的显微镜
Read Article