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JoVE Journal
Bioengineering

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荧光技术结合测定活细胞中的力敏感蛋白动力学
 
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荧光技术结合测定活细胞中的力敏感蛋白动力学

Article DOI: 10.3791/58619
November 2nd, 2018

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Summary November 2nd, 2018

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在这里, 我们提出了一个协议, 同时使用 förster 共振能量转移的张力传感器来测量光漂白后的蛋白质负荷和荧光恢复, 以测量蛋白质动力学, 从而能够测量对力敏感的测量活细胞内的蛋白质动力学。

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