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荧光技术结合测定活细胞中的力敏感蛋白动力学
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques
DOI:

08:28 min

November 02, 2018

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Chapters

  • 00:04Title
  • 00:43Generate Samples for Imaging
  • 02:13Set up Microscope for Imaging
  • 03:57Acquire FRET-FRAP Data
  • 06:53Results: Vinculin Force-Sensitive Dynamics at the Focal Adhesion
  • 07:52Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个协议, 同时使用 förster 共振能量转移的张力传感器来测量光漂白后的蛋白质负荷和荧光恢复, 以测量蛋白质动力学, 从而能够测量对力敏感的测量活细胞内的蛋白质动力学。

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