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Journal
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Bioengineering
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荧光技术结合测定活细胞中的力敏感蛋白动力学
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques
Please note that all translations are automatically generated.
Click here for the English version.
荧光技术结合测定活细胞中的力敏感蛋白动力学
DOI:
10.3791/58619-v
•
08:28 min
•
November 02, 2018
•
Katheryn E. Rothenberg
,
Ishaan Puranam
,
Brenton D. Hoffman
1
Department of Biomedical Engineering
,
Duke University
Chapters
00:04
Title
00:43
Generate Samples for Imaging
02:13
Set up Microscope for Imaging
03:57
Acquire FRET-FRAP Data
06:53
Results: Vinculin Force-Sensitive Dynamics at the Focal Adhesion
07:52
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个协议, 同时使用 förster 共振能量转移的张力传感器来测量光漂白后的蛋白质负荷和荧光恢复, 以测量蛋白质动力学, 从而能够测量对力敏感的测量活细胞内的蛋白质动力学。
Tags
Force-sensitive Protein Dynamics
Fluorescent Techniques
Mechanobiology
Vinculin
Talin
Cadherins
Nesprin
Tension Sensor Construct
Fibronectin
FRAP Laser
Acceptor Fluorophore
Mechanically-sensitive Proteins
Cell-cell Interactions
Cell-environment Interactions
Molecular Scale
Living Cells
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