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利用磁性纳米粒子丰富和扩大罕见抗原特异性 t 细胞

JoVE Journal
Engineering

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JoVE Journal Engineering

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

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利用磁性纳米粒子丰富和扩大罕见抗原特异性 t 细胞

DOI:

10.3791/58640-v

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09:28 min

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November 17, 2018

•

John W. Hickey^2,3,4, Jonathan P. Schneck⁴

¹Department of Biomedical Engineering, School of Medicine,Johns Hopkins University, ²Institute for Cell Engineering, School of Medicine,Johns Hopkins University, ³Institute for Nanobiotechnology,Johns Hopkins University, ⁴Department of Pathology, School of Medicine,Johns Hopkins University

Chapters

00:04Title
01:27Artificial Antigen Presenting Cell (aAPC) Nanoparticle Formation
03:18Antigen-Specific CD8+ T Cell Enrichment and Expansion
06:59Results: Representative Enrichment and Expansion of Antigen-Specific CD8+ T Cells
08:45Conclusion

Summary

Automatic Translation

抗原特异性 t 细胞由于其频率极低, 很难在治疗中表征或利用。在此, 我们提供了一个协议, 以开发一个磁性粒子, 它可以结合到抗原特异性 t 细胞, 以丰富这些细胞, 然后扩大他们的几百倍, 用于表征和治疗。

Tags

Magnetic Nanoparticles Antigen-specific T Cells Enrichment Expansion Neoepitopes Artificial Antigen-presenting Cells Immunotherapy Cellular Therapy Resuspension Buffer Stimulatory Signals Quenching Buffer

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