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Journal
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Genetics
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qkat: 杀手细胞免疫球蛋白样受体基因的定量半自动分型
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes
Please note that all translations are automatically generated.
Click here for the English version.
qkat: 杀手细胞免疫球蛋白样受体基因的定量半自动分型
DOI:
10.3791/58646-v
•
07:58 min
•
March 06, 2019
•
Jyothi Jayaraman
2,3,4
,
Vitalina Kirgizova
,
Da Di
5
,
Christopher Johnson
6
,
Wei Jiang
7
,
James A. Traherne
1
Department of Pathology
,
University of Cambridge
,
2
Department of Physiology, Development and Neuroscience
,
University of Cambridge
,
3
Department of Obstetrics and Gynaecology
,
University of Cambridge School of Medicine, NIHR Cambridge Biomedical Research Centre
,
4
Centre for Trophoblast Research
,
University of Cambridge
,
5
Department of Genetics & Evolution
,
University of Geneva
,
6
Royal Papworth Hospital
,
7
Department of Plant Sciences
,
University of Cambridge
Chapters
00:04
Title
01:45
Preparation and Plating Out of DNA
02:25
Preparation of the Master Mix
03:36
qPCR Assay
04:07
Post-run Analysis
05:19
Export of Results and Copy Number Calculations
06:39
Results: qKAT Used to Copy Number Type KIR Genes
06:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
定量杀伤细胞免疫球蛋白样受体 (kir) 半自动分型 (qkat) 是一种简单、高通量、经济高效的方法, 用于复制数字型
kir
基因, 用于人群和疾病关联研究。
Tags
QKAT
Quantitative KIR Automated Typing
Killer-cell Immunoglobulin-like Receptor
NK Cell Receptor Genetics
Haplotype Diversity
Disease Association
High-throughput
Gene Copy Number
Multiplex PCR
Real-time PCR
Relative Quantification
Population Genetics
HLA
Viral Infections
Cancer
Stem Cell Transplantation
Pregnancy Disorders
Article
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