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Journal
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Medicine
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板基检测血液中一氧化氮的 vasp 磷酸化
JoVE Journal
Medicine
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JoVE Journal
Medicine
Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation
Please note that all translations are automatically generated.
Click here for the English version.
板基检测血液中一氧化氮的 vasp 磷酸化
DOI:
10.3791/58647-v
•
07:13 min
•
January 07, 2019
•
Sirada Srihirun
2
,
Alan N. Schechter
,
Barbora Piknova
1
Department of Pharmacology, Faculty of Dentistry
,
Mahidol University
,
2
Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases
,
National Institutes of Health
Chapters
00:04
Title
00:45
Blood Sample Preparation and Deoxygenation
02:52
Red Blood Cell (RBC) Nitrite Reduction and Vasodilator-stimulated Phosphoprotein (VASP) Western Blotting
04:39
Results: Representative Platelet-based Nitric Oxide (NO) Detection
06:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了一个协议, 以解决潜在的使用血小板作为一个高度敏感的一氧化氮传感器在血液中。它描述了最初的血小板制备和使用亚硝酸盐和脱氧红细胞作为一氧化氮发生器。
Tags
Platelet
Nitric Oxide
VASP Phosphorylation
Blood
In Vitro
In Vivo
P-VASP
Sensor
Centrifugation
Platelet-rich Plasma
Tyrode Buffer
Red Blood Cells
Deoxygenation
Nitrite Reduction
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