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用不连续密度梯度用胶囊分离细菌

JoVE Journal
Genetics

This content is Free Access.

JoVE Journal Genetics

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

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用不连续密度梯度用胶囊分离细菌

DOI:

10.3791/58679-v

•

05:52 min

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January 07, 2019

•

Theresa Feltwell, Matthew J. Dorman, David A. Goulding, Julian Parkhill, Francesca L. Short²

¹Wellcome Sanger Institute, Wellcome Genome Campus, ²Department of Medicine,University of Cambridge

Chapters

00:04Title
00:48Preparation of Bacterial Strains of Mutant Libraries
01:40Preparation of Gradient Dilutions and Mini-gradient Test
02:39Preparation of Discontinuous Density Gradients
03:39Adding Prepared Cells to Gradients and Separation by Centrifuge
04:30Results: Density Gradient Centrifugation Used to Separate Bacterial Strains Based on Capsule Production
05:10Conclusion

Summary

Automatic Translation

我们展示了使用不连续密度梯度来根据胶囊生产分离细菌种群。该方法用于比较培养物间的胶囊数量, 分离具有特定胶囊表型的突变体, 或鉴定胶囊调节剂。这里描述的是该分析的优化和运行。

Tags

– Bacteria Separation- Capsule Amount- Discontinuous Density Gradient- Klebsiella- Capsule Regulation- Visual Demonstration- Density Gradient Medium- Centrifugation- Cell Suspension- Gradient Dilutions- Gradient Interface

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