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用不连续密度梯度用胶囊分离细菌
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient
DOI:

05:52 min

January 07, 2019

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Chapters

  • 00:04Title
  • 00:48Preparation of Bacterial Strains of Mutant Libraries
  • 01:40Preparation of Gradient Dilutions and Mini-gradient Test
  • 02:39Preparation of Discontinuous Density Gradients
  • 03:39Adding Prepared Cells to Gradients and Separation by Centrifuge
  • 04:30Results: Density Gradient Centrifugation Used to Separate Bacterial Strains Based on Capsule Production
  • 05:10Conclusion

Summary

Automatic Translation

我们展示了使用不连续密度梯度来根据胶囊生产分离细菌种群。该方法用于比较培养物间的胶囊数量, 分离具有特定胶囊表型的突变体, 或鉴定胶囊调节剂。这里描述的是该分析的优化和运行。

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