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Journal / Immunology and Infection / Marquage immunohistochimique Fluorescent…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Marquage immunohistochimique Fluorescent multiplexé, l’imagerie et l’analyse des échantillons histologique du lymphome
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Marquage immunohistochimique Fluorescent multiplexé, l’imagerie et l’analyse des échantillons histologique du lymphome

Article DOI: 10.3791/58711-v • 07:52 min • January 9th, 2019
January 9th, 2019 •
*1,2, *3, 3, 2, 2, 2, 4, 4, 2,3, 2,4
1Department of Laboratory Medicine, AnSteel Group General Hospital, 2Cancer Science Institute of Singapore, National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, 4Department of Haematology-Oncology, National University Health System
* These authors contributed equally

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Nous décrivons ici un protocole pour multiplex immunohistochemical fluorescent de coloration et d’imagerie pour la localisation simultanée de plusieurs antigènes associés aux cancer des lymphomes. Ce protocole peut être étendu à l’analyse de colocalisation de biomarqueurs dans toutes les coupes de tissus.

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Immunologie et Infection numéro 143 immunofluorescence multiplexé multispectral imaging analyse quantitative biomarqueurs lymphome pathologie numérique
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