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Multiplex fluorescerende Immunohistochemical flekker, bildebehandling og analyse i histologiske prøver lymfom
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
DOI:

07:52 min

January 09, 2019

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Chapters

  • 00:04Title
  • 00:57Monoplex Tyramide-Based Immunofluorescence
  • 03:22Monoplex Slide and Tissue Microarray (TMA) Scanning
  • 04:36Data Analysis
  • 06:28Results: Representative Mutiplexed Fluorescent Immunohistochemical (mf-IHC) Staining, Imaging, and Analysis
  • 07:10Conclusion

Summary

Automatic Translation

Her beskriver vi en protokoll for multiplex fluorescerende immunohistochemical flekker og tenkelig den samtidige lokalisering av flere kreft-assosiert antigener i lymfom. Denne protokollen kan utvides til colocalization analyse av biomarkers i alle vev inndelinger.

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