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Multiplex fluorescerende immunhistokemisk farvning, billedbehandling og analyse i histologiske prøver af lymfom
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
DOI:

07:52 min

January 09, 2019

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Chapters

  • 00:04Title
  • 00:57Monoplex Tyramide-Based Immunofluorescence
  • 03:22Monoplex Slide and Tissue Microarray (TMA) Scanning
  • 04:36Data Analysis
  • 06:28Results: Representative Mutiplexed Fluorescent Immunohistochemical (mf-IHC) Staining, Imaging, and Analysis
  • 07:10Conclusion

Summary

Automatic Translation

Her beskriver vi en protokol for multiplex fluorescerende immunhistokemisk farvning og imaging for simultan lokalisering af flere kræft-associerede antigener i lymfom. Denne protokol kan udvides til en colocalization analyse af biomarkører inden for alle væv sektioner.

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