Journal
/
Immunology and Infection
/
Multiplex fluorescerende immunhistokemisk farvning, billedbehandling og analyse i histologiske prøver af lymfom
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Multiplex fluorescerende immunhistokemisk farvning, billedbehandling og analyse i histologiske prøver af lymfom

DOI:
10.3791/58711-v

•

07:52 min

•

January 09, 2019

•

, , , , , , , , ,
1Department of Laboratory Medicine,AnSteel Group General Hospital, 2Cancer Science Institute of Singapore,National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine,National University of Singapore, 4Department of Haematology-Oncology,National University Health System

Chapters

  • 00:04Title
  • 00:57Monoplex Tyramide-Based Immunofluorescence
  • 03:22Monoplex Slide and Tissue Microarray (TMA) Scanning
  • 04:36Data Analysis
  • 06:28Results: Representative Mutiplexed Fluorescent Immunohistochemical (mf-IHC) Staining, Imaging, and Analysis
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi en protokol for multiplex fluorescerende immunhistokemisk farvning og imaging for simultan lokalisering af flere kræft-associerede antigener i lymfom. Denne protokol kan udvides til en colocalization analyse af biomarkører inden for alle væv sektioner.

Tags

Multiplexed Fluorescent ImmunohistochemistryLymphomaTumor MicroenvironmentAntigen Co-expressionSpatial RelationshipAntigen RetrievalMicrowave StrippingPeroxidase BlockTyramide-based Fluorescent ReagentSpectral Imaging

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice

Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples

Multiplex Påvisning af Bakterier i komplekse kliniske og miljøprøver hjælp Oligonucleotid koblede Fluorescent Mikrokugler

Brug fluorescerende proteiner til at visualisere og kvantificere<em> Chlamydia</em> Vakuole Vækst Dynamics i levende celler

Neutrofil Isolering og analyse for at fastslå deres rolle i lymfom Cell Følsomhed til terapeutiske midler

Ikke-invasiv<em> In vivo</em> Fluorescens optisk billeddannelse af inflammatorisk MMP Aktivitet Brug et aktiverbart Fluorescent Imaging Agent

Meget multiplexeret, superopløselig billeddannelse af T-celler ved hjælp af madSTORM

Anvendelse af kapsler til negativ farvning af virale prøver inden for biokonduktion

Histologiske kvantificering at bestemme lunge svampe byrde i eksperimentel aspergillose

Ved hjælp af robotsystemer til at behandle og integrere Colon Murine prøver for histologiske analyser

Read Article