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A Spheroid Killing Assay by CAR T Cells
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Cancer Research
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JoVE Journal Cancer Research
A Spheroid Killing Assay by CAR T Cells

A Spheroid Killing Assay by CAR T Cells

DOI:
10.3791/58785-v

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08:19 min

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December 12, 2018

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, , ,
1Department of Cellular Therapy, Department of Oncology,Oslo University Hospital-Radiumhospitalet

Chapters

  • 00:04Title
  • 00:31Colorectal Cancer Cell Line Spheroid Generation
  • 03:113D Tumor Spheroid Killing Assay
  • 05:00Automated Image Analysis
  • 06:59Results: Representative Spheroid Killing Analysis
  • 07:54Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol is designed to assess immunotherapeutic redirected T-cell (CAR T-cell) cytotoxicity against 3D structured cancerous cells (spheroids) in real time.

Tags

Keywords: 3D Tumor CellsCAR T CellsCytotoxicity AssayColorectal CancerSpheroidAutomated ImagingTime-lapse MicroscopyImmunotherapy

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