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微胶质过程对急性脑片 atp 或血清素的双光子成像
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices
DOI:

07:27 min

January 31, 2019

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Chapters

  • 00:04Title
  • 00:42Dissection Area Preparation
  • 01:51Brain Slicing
  • 03:112-photon Live Imaging
  • 04:12Compound Application
  • 06:14Results: Microglial Response Analyses
  • 07:05Conclusion

Summary

Automatic Translation

小胶质细胞是大脑中的常驻免疫细胞, 随着环境变化的形态变化而迅速反应。该协议描述了如何使用双光子显微镜研究微胶质过程对血清素或 atp 的吸引力, 在急性脑切片的小鼠。

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