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微胶质过程对急性脑片 atp 或血清素的双光子成像
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices
Please note that all translations are automatically generated.
Click here for the English version.
微胶质过程对急性脑片 atp 或血清素的双光子成像
DOI:
10.3791/58788-v
•
07:27 min
•
January 31, 2019
•
Fanny Etienne
2,3
,
Vincenzo Mastrolia
2,3
,
Luc Maroteaux
2,3
,
Jean-Antoine Girault
2,3
,
Nicolas Gervasi*
1,2,3
,
Anne Roumier*
1,2,3
1
Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France
,
2
Sorbonne Université, Paris, France
,
3
Institut du Fer à Moulin, Paris, France
Chapters
00:04
Title
00:42
Dissection Area Preparation
01:51
Brain Slicing
03:11
2-photon Live Imaging
04:12
Compound Application
06:14
Results: Microglial Response Analyses
07:05
Conclusion
Summary
Automatic Translation
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Automatic Translation
小胶质细胞是大脑中的常驻免疫细胞, 随着环境变化的形态变化而迅速反应。该协议描述了如何使用双光子显微镜研究微胶质过程对血清素或 atp 的吸引力, 在急性脑切片的小鼠。
Tags
Two-photon Imaging
Microglial Processes
ATP
Serotonin
Acute Brain Slices
Directional Motility
3D Print Interface
Perfusion Chambers
Choline ACSF
Vibrating Slicer
Interface Chamber
Peristaltic Pump
Recording Chamber
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