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Forberedelse af rytmisk-aktiv i Vitro Neonatal gnaver hjernestammen-rygmarven og tynd skive
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Neuroscience
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JoVE Journal Neuroscience
Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice

Forberedelse af rytmisk-aktiv i Vitro Neonatal gnaver hjernestammen-rygmarven og tynd skive

DOI:
10.3791/58870-v

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06:32 min

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March 23, 2019

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, , ,
1Center for Perinatal Biology, Department of Basic Sciences,Loma Linda University, 2Department of Biology,The College of New Jersey, 3Department of Neurosurgery,Loma Linda University

Chapters

  • 00:04Title
  • 00:41Neuraxis Isolation
  • 03:59Histological Preparation
  • 05:15Results: Representative Recordings from En Bloc and Slice Preparations
  • 05:52Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol både visuelt kommunikerer hjernestammen rygmarven forberedelse og præciserer forberedelse af hjernestammen tværgående skiver på en omfattende måde, trin for trin. Det var designet til at øge reproducerbarhed og øge sandsynligheden for at få levedygtigt, langtidsholdbare, rytmisk-aktive skiver til optagelse af neurale output fra regionerne respiratorisk af hjernestammen.

Tags

Rhythmically-activeIn VitroNeonatalRodentBrainstem-spinal CordThin SliceDissectionMirian Medullary Respiratory NetworkElectrophysiologyNeural Control CircuitBreathing RhythmsGenetically Modified RodentsDorsal LaminectomyVentral LaminectomyPonsMedullaSpinal Cord

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