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胚胎小鼠神经干细胞的分离与培养

JoVE Journal
Neuroscience
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JoVE Journal Neuroscience

Isolation and Culture of Embryonic Mouse Neural Stem Cells

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胚胎小鼠神经干细胞的分离与培养

DOI:

10.3791/58874-v

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09:04 min

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November 11, 2018

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Farshad Homayouni Moghadam, Maryam Sadeghi-Zadeh³, Bahareh Alizadeh-Shoorjestan³, Reza Dehghani-Varnamkhasti³, Sepideh Narimani², Leila Darabi³, Abbas Kiani Esfahani, Mohammad Hossein Nasr Esfahani

¹Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology,ACECR, ²Department of Biology, Faculty of Basic Sciences,Islamic Azad University, ³Department of Biology,ACECR Institute of Higher Education

Chapters

00:00Title
01:18Animal Surgery and Micro-Dissection
02:51Bending the Tip of the Needle
03:12Isolation of E13 Mouse Brain and Micro-Dissection
06:32Trypsination and Passaging of Neurospheres
07:46Representative Results
08:42Conclusion

Summary

Automatic Translation

在这里, 我们介绍了一种新的显微外科技术, 从 e¹³小鼠胚胎神经节隆起分离神经干细胞。

Tags

Keywords: Embryonic Mouse Neural Stem Cell Isolation Culture Ganglionic Eminence Micro-dissection Single Cell Suspension HEPES MEM Buffer Dissociation Suspension Culture Nerve Cells

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