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Lucifer Yellow – A Robust Paracellular Permeability Marker in a Cell Model of the Human Blood-brain Barrier
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Bioengineering
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JoVE Journal Bioengineering
Lucifer Yellow – A Robust Paracellular Permeability Marker in a Cell Model of the Human Blood-brain Barrier

Lucifer Yellow – A Robust Paracellular Permeability Marker in a Cell Model of the Human Blood-brain Barrier

DOI:
10.3791/58900-v

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06:22 min

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August 19, 2019

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, , ,
1Department of Pharmaceutical, Administrative and Social Sciences,Duquesne University, 2Department of Pharmaceutical Sciences,University of Kentucky

Chapters

  • 00:04Title
  • 00:57Cell Plating
  • 02:28Lucifer Yellow Apparent Permeability Assay
  • 04:26Results: Apparent Kinetics of Tight Junction Barrier Formation in hCMEC/D3 cells
  • 05:39Conclusion

Summary

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Automatic Translation

We present a fluorescence assay to demonstrate that Lucifer Yellow (LY) is a robust marker to determine the apparent paracellular permeability of hCMEC/D3 cell monolayers, an in vitro model of the human blood-brain barrier. We used this assay to determine the kinetics of a confluent monolayer formation in cultured hCMEC/D3 cells.

Tags

Lucifer YellowParacellular PermeabilityBlood-brain BarrierCell ModelTight JunctionsApparent PermeabilityCell PlatingCollagen CoatingHCMEC/D3 CellsCell ConfluencyLucifer Yellow AssayTransport BufferFluorescence Measurement

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