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Anhebung der mexikanischen Tetra Astyanax Mexicanus für Analyse der Post-larval Phänotypen und vollständig-hängen Immunohistochemistry
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
Raising the Mexican Tetra Astyanax mexicanus for Analysis of Post-larval Phenotypes and Whole-mount Immunohistochemistry
DOI:

06:42 min

December 28, 2018

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Chapters

  • 00:04Title
  • 00:38Breeding
  • 01:20Hatching Fertilized Eggs
  • 02:16Transfer of Hatched Larvae to Nursery Containers
  • 02:45Preparation of Rotifer-based Fish Food and Feeding of Post-Larval Fish
  • 03:19Whole-Mount Immunohistochemistry of Post-Larval Fish
  • 05:11Results: Surface Fish Produce Greatest Number of Larvae per Spawn
  • 05:56Conclusion

Summary

Automatic Translation

In diesem Protokoll wir zeigen, wie Astyanax Mexicanus Erwachsene zu züchten, die Larven zu erhöhen, und ganze-Mount Immunohistochemistry auf Post-Larven Fische zu vergleichen die Phänotypen der Oberfläche und Höhle Morphotypen führen.

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