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Genetics

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Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
 

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II

Article DOI: 10.3791/56736
February 26th, 2018

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Summary February 26th, 2018

Oogenesis in mammals is known to be error-prone, particularly due to chromosome missegregation. This manuscript describes chromatin spread preparation methods for mouse prophase, metaphase I and II-staged oocytes. These fundamental techniques allow for the study of chromatin-bound proteins and chromosome morphology throughout mammalian oogenesis.

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