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En simpel Migration/Invasion arbejdsgang ved hjælp af en automatiseret Live-celle Imager
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager
DOI:

09:17 min

February 02, 2019

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Chapters

  • 00:04Title
  • 00:51Optimal Migration Assay Seeding Density
  • 02:49Migration and Invasion Assay Preparation
  • 03:36Wound Scratch
  • 05:35Wound Assay Imaging and Data Analysis
  • 06:43Results: Representative Migration and Invasion Assay Analyses
  • 08:22Conclusion

Summary

Automatic Translation

Den nuværende protokol beskriver en integreret metode undersøger kræft celle migration og invasion på en enkelt platform i realtid, som giver en let gengivelig og tid-effektiv mulighed for at studere cellen mobilitet og morfologi.

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